By John H. Hash
The severely acclaimed laboratory regular, tools in Enzymology, is among the such a lot hugely revered courses within the box of biochemistry. due to the fact that 1955, every one quantity has been eagerly awaited, often consulted, and praised by means of researchers and reviewers alike. The sequence includes a lot fabric nonetheless suitable this day - actually an important ebook for researchers in all fields of lifestyles sciences.
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21T. Ichikawa, M. Date, T. Ishikura, and A. Osaki, Folia Microbiol. (Prague) 16, 218 (1971). 22R. C. Pittenger and E. McCoy, J. Bacteriol. 65, 56 (1953). 38 METHODS FOR THE STUDY OF ANTIBIOTICS  advantageous by some investigators in selecting improved antibiotic producers; others have reported poor correlation when isolates selected by this method were eventually tested in shaker flasks. In any case, the following suggestions will help avoid pitfalls: (a) If the antibiotic is not bactericidal, the colonies to be overlayered should first be replica-plated 2~ to another set of plates before flooding with the test organism.
This method will produce fine suspensions devoid of large particles. 6. For certain asporogenous fungi, particularly those exhibiting a rapid filamentous growth, the hyphal tips may be exposed to a mutagen more advantageously than the entire colony growing on a plate. This may be readily accomplished by placing a sterile square of cellophane (avoid water-proofed cellophane) or other porous membrane 3-4 cm wide on the agar surface of a nutrient medium; the center of the square is inoculated with the fungus, and, after an appropriate period of incubation, the growing front of the colony will grow beyond the edge of the cellophane.
III. IV. V. Introduction . . . . . . . . . Fermentation Conditions . . . . . . . . Assay for Antibiotic Yield . . . . . . . Preparation of Suspension for Mutation Treatment . . . . Mutation Techniques . . . . . . . . A. Ultraviolet Light (UV) Irradiation . . . . . . B. Ionizing Radiation . . . . . . . . C. Alkylating Agents . . . . . . . . D. Other Mutagens . . . . . . . . E. Combined Action . . . . . . . . VI. Selection from Mutagen-Treated Populations .